the effect of wild type p53 gene transfer on growth properties and tumorigenicity of panc-1 tumor cell line

نویسندگان

مرضیه حقی شریفیا تقوی

marzieh hagi-sharifia taghavi جمشید داوودی

jamshid davoodi منوچهر میرشاهی

manouchehr mirshahi

چکیده

the p53 protein function is essential for the maintenance of the nontumorigenic cell phenotype. pancreatic tumor cells show a very high frequency of p53 mutation. to determine if restoration of wild type p53 function can be used to eliminate the tumorigenic phenotype in these cells, pancreatic tumor cell lines, panc-1 and htb80, differing in p53 status were stably transfected with exogenous wild type p53 gene. methods: the transfection was performed using polybrene/dmso-assisted gene transfer method. the wild type p53 gene integration into genomic dna was detected by southern blot and pcr. furthermore, the expression of wild type p53 protein was detected in selected clones by immunohistochemistry and western blot. results: while htb80 cell line failed to produce a stable p53 expressing clone, the panc-1 cells produced stable lines. following characterization of clones, the growth rate and tumorigenicity of panc-1 wild type p53 clones were compared to the control cells. our data showed that the expression of wild type p53 decreased the growth rate of panc-1 cells. it was also observed that the expression of wild type p53 in panc-1 cells suppressed its potential for tumor formation in nude mice, completely, while the parental line leads to the formation of a relatively large tumor. conclusion: our results suggest that gene therapy based on restoration of wild type p53 protein function in pancreatic tumor cells with high amount of mutant p53 is a feasible option in pancreatic cancer treatment.

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منابع مشابه

The Effect of Wild Type P53 Gene Transfer on Growth Properties and Tumorigenicity of PANC-1 Tumor Cell Line

The p53 protein function is essential for the maintenance of the nontumorigenic cell phenotype. Pancreatic tumor cells show a very high frequency of p53 mutation. To determine if restoration of wild type p53 function can be used to eliminate the tumorigenic phenotype in these cells, pancreatic tumor cell lines, PANC-1 and HTB80, differing in p53 status were stably transfected with exogenous wil...

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The effect of wild type P53 gene transfer on growth properties and tumorigenicity of PANC-1 tumor cell line.

BACKGROUND The p53 protein function is essential for the maintenance of the nontumorigenic cell phenotype. Pancreatic tumor cells show a very high frequency of p53 mutation. To determine if restoration of wild type p53 function can be used to eliminate the tumorigenic phenotype in these cells, pancreatic tumor cell lines, PANC-1 and HTB80, differing in p53 status were stably transfected with ex...

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Wild Type p53 Gene Transfer Increases Chemosensitivity and Apoptotic Response of PANC-1 Pancreatic Tumor Cell Line

The effect of p53 gene therapy on chemosensitivity and apoptotic response of PANC-1 tumor cells, which express high amount of mutant p53, to cancer chemotherapeutic agents of Etoposide and Doxorubicin was investigated. Comparison of the chemosensitivity of PANC-1 cells to its wild type p53 transfectants showed that wt-p53 expressing transfectants are more sensitive to both Etoposide and Doxorub...

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عنوان ژورنال:
iranian biomedical journal

جلد ۱۱، شماره ۱، صفحات ۱-۶

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